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閱讀：428發(fā)布時間：2013-4-17
Rabbit Anti-PECAM-1 （ CD31 antigen ）抗體說明書
From: RICKY
Cat. Number: Rs-0468R
Quantity size: 0.2ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: This protein is a cell adhesion molecule expressed on plaets and at endothelial cell intercellular junctions. Type I membrane protein. SIZE: 738 amino acids; 82536 Da. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart. PTM: Phosphorylated on Ser and Tyr residues after cellular activation. SIMILARITY: Contains 6 Ig-like C2-type （immunoglobulin-like） domains.
Specificity: · anti- PECAM-1 is a rabbit monoclonal antibody unconjugated
·specific for PECAM-1 of human, mouse and rat
· epitope mapping near the amino terminus of PECAM-1 of
human, mouse and rat origin
· use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 82kDa
Application: · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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